Ugh. Ok. "Pissed off somewhat" does not describe what I'm feeling. More like, "super pissed off".
First of all, my experiment failed. AGAIN. And I can't quite figure what's wrong. And since you are supposed to be our mentor, I thought I could ask for your opinion or advice. Turns out, not much of what you said makes sense.
First of all, you said that I didn't prepare my antibody mixture earlier. Hello. You're the one that told me that the antibody degrades very quickly. Next, you want me to prepare the antibody mixture one hour before adding it to the slides. And now, you scold me for preparing it 5-10 minutes before adding it to the slides, because I didn't see the point of preparing it so damn early for it to degrade so quickly. So what? Am I supposed to apologise for not brainlessly following orders? And by right, since I prepared it later, shouldn't the staining of the antibody be stronger? =/
Next, you accuse me of extending the timings that I shouldn't be extending. Oi. The only timings I increased is leaving my damn tissues in the buffer solution. Which you're the one that told us it should not affect the results, because the function of the buffer is to wash away excess reagents and to keep the cells from being dry.
If you're going to accuse me of screwing up the procedure, at least give me some reason why what I was doing was wrong.
Ugh. You are no help at all.
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And thanks to my mentor's "wonderful" help, I decided to go home and figure out what went wrong myself. I think I would be more productive.
And I was right.
Anyway, my first mistake was probably adding the blocking agent to the antibody mixture first. =/ After reading through the protocol again, I realised that the blocking agent was meant to block out excess Ig reagent... Which the primary antibody was supposed to bind to... So... If I block everything, there won't be any expression. =/ I feel like such a genius.
And my second mistake... Let's just say that the journal articles I've been reading just keep saying it's found in the brain, it's found in the brain. And only now I realise that when the articles said "brain", they mean "cerebellum". I've been testing the hemisphere for the protein. Whoo.
I really am a freaking genius. =/ No wonder I can't get anything.
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Oh ya! I just had this most epic conversation with Tamaki today. XD He has taken to calling his apartment in Beijing his Pimp House. XD Apparently, he plans to pimp out all the guys in the apartment while he collects entrance fees. XD
Haha! He wanted Spike to be his poster boy for the house. Haha! I suggested Hammer instead. =P Best of all, Kitty agreed to sell out her boyfriend provided Tamaki gives her 80% of the earnings. XD Hahaha! And of course, in exchange, Kitty has to "bring in" the only other guy in class that Tamaki likes and is not in his apartment. (*coughacertaintalldudesittingatthebackrowcough*) The conversation is epic funny!
And of course, Tamaki has great plans for the selling of Kyoya himself. XD Haha! So watch out Kyoya! XD
And now, Tamaki is all hyped up to go China. I don't wonder why. XD
P.S. I helped with his ideas too. XD It was too amusing not to.